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KMID : 1140120110160010019
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Cancer Prevention Research
2011 Volume.16 No. 1 p.19 ~ p.25
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Effect of Bi-1 Overexpression on Mitochondria in Human Fibrosarcoma Cells
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Kwon Jee-Young
Kim Jung-Hyun
Jeoung Seok-Won
Lee Young-Sik
Seo Young-Rok
Cho Ssang-Goo
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Abstract
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The antiapoptotic protein Bax inhibitor-1 (Bi-1) was identified through a functional yeast screening designed to select for human cDNAs that inhibit Bax-induced apoptosis. Although Bi-1 has been known as a protein to have anti-apoptotic function against endoplasmic reticulum (ER) stress via regulation of Ca2+ levels between ER and cytosol, the role of Bi-1 in mitochondira was not clarified. In this study, we found that Bi-1 localized in both mitochondria and endoplasmic reticulum. Therefore, we investigated the effect of Bi-1 on the morphological alteration of mitochondria as well as ER using electron microscope (EM). Our data showed the morphological change of mitochondria and alteration of ER generation upon Bi-1 overexpression, implicating that Bi-1 might play an important role in mitochondria-mediated apoptosis. Taken together, we suggest that the study using electron microscopy might provide a new aspect in the therapy of diseases caused by abnormal apoptosis regulation.
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KEYWORD
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Apoptosis, Bax inhibitor-1 (Bi-1), Mitochondria
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